Title of article :
Evaluation of rs7903146 Polymorphism in TCF7L2 Gene among Type 2 Diabetes Patients
Author/Authors :
Momen Maragheh ، Salar Department of Biotechnology - Biotechnology Research Center (BRC), Pasteur Institute of Iran - Islamic Azad University, Central Tehran Branch , Aslani Zakariya ، Alireza Department of Chemistry - Faculty of Science - Ataturk University , Ghaedi ، Elham Department of Medicine - Pirogov Russian National Research Medical University , Saeifar ، Fahimeh Department of Midwifery - Health Center of Maragheh , Navid Talemi ، Mahdi Department of Pharmacology - Guilan University of Medical Sciences , Rezanezhad Varjovi ، Mehran Department of Microbiology - Islamic Azad University of Maragheh , Bagheri ، Mahdi Department of Medicine - Medical University of Ilam
Abstract :
Background: Diabetes is characterized by decreased insulin sensitivity and is a growing health threat worldwide. Therefore, this study aimed to evaluate the relationship between rs7903146 polymorphism in the TCF7L2 gene with type 2 diabetes in East Azerbaijan province, Iran. Materials and method: A total of 100 blood samples were collected from diabetic and healthy people. The samples were subjected to specific primers, PCR, and electrophoresis after DNA extraction from all samples and quality control. Finally, the PCR products were treated with RSal restriction enzyme and electrophoresed again, and the target polymorphism was checked. Results: The frequency of genotypes were TT=33.7%, CC=16.8% and CT=49.5% for healthy people and TT=43%, CC=14% and TC=43% in diabetics. The percentage of the T allele in healthy and diabetic people was as much as 42.1% and 64.5%, respectively. Conclusion: According to the results, there was probably a relationship between rs7903146 polymorphism in the TCF7L2 gene and diabetes.
Keywords :
Polymorphism , rs7903146 , TCF7L2 , Type 2 diabetes
Journal title :
International Journal Of Medical Investigation
Journal title :
International Journal Of Medical Investigation
