Title of article :
Association between Tissue Expression of Toll- Like Receptor and Some Clinicopathological Indices in Oral Squamous Cell Carcinoma
Author/Authors :
Zare ، Reza Oral and Maxillofacial Diseases Research Center - Mashhad University of Medical Sciences , Anvari ، Kazem Department of Radiotherapy Oncology - Cancer Research Center - Mashhad University of Medical Sciences , Mohajertehran ، Farnaz Department of Oral and Maxillofacial Pathology - Oral and Maxillofacial Diseases Research Center, School of Dentistry - Mashhad University of Medical Sciences , Farshbaf ، Alieh Dental Research Center - Mashhad University of Medical Sciences , Pakfetrat ، Atessa Oral and Maxillofacial Diseases Research Center - Mashhad University of Medical Sciences , Ansari ، Amir Houshang Student research committee - Mashhad University of Medical Sciences , Ghelichli ، Maryam Department of Oral and Maxillofacial Pathology - School of Dentistry - Golestan University of Medical Sciences , Mohtasham ، Nooshin Oral and Maxillofacial Diseases Research Center - Mashhad University of Medical Sciences
Abstract :
Background: The oral squamous cell carcinoma (OSCC) composes about 90% of all head and neck cancers. The toll-like receptor (TLR)+ immune cells have potential of invasion and malignancy transformation. The aim of this study was assessment of possible associations between clinicopathological indices and TLR2 and TLR9 gene expression in OSCC. Methods: Forty-two OSCC samples with related healthy margins including 25 early and 17 advanced stages were gathered. The samples were classified histologically from grade I to II. The expression of TLR2 and TLR2 was evaluated by Real-time PCR. The patient’s disease-free survival (DFS) and overall survival (OS) were analyzed using SPSS V.23 software. Results: The expression of TLR2 and TLR9 genes in tumor tissues (especially in grade I and II) were higher than healthy surgical margin tissue (p 0.001). TLR9 expression in grade II was statistically significant than grade I in tumor tissue (p 0.001). TLR9 expression in advanced stage was statistically significant in compare to early stage (p= 0.012). In advanced stage both overall survival (p= 0.029) and disease-free survival (p= 0.012) were statistically lower than early stage. The follow-up time to recurrence in advanced stage was statistically lower than early stage (p= 0.007). Conclusions: Overexpression of TLRs 2, 9 play role in the pathogenesis and tumor development of OSCC and can be applied as biomarker in prognostic approaches.
Keywords :
Disease , free survival , Oral squamous cell carcinoma , Overall survival , TLR2 , TLR9
Journal title :
Reports of Biochemistry and Molecular Biology (RBMB)
Journal title :
Reports of Biochemistry and Molecular Biology (RBMB)