Title of article :
Novel RNA extraction method from human tears
Author/Authors :
Dara ، Mahintaj Stem Cells Technology Research Center - Shiraz University of Medical Sciences , Habibi ، Azam Department of tissue engineering and cell therapy - School of Advanced Technology in Medicine - Shiraz University of Medical Sciences , Azarpira ، Negar Transplant Research Center - Shiraz University of Medical Sciences , Dianatpour ، Mehdi Department of Medical Genetics - Stem Cells Technology Research Center - Shiraz University of Medical Sciences , Nejabat ، Mahmood Department of Ophthalmology - Poostchi Ophthalmology Research Center, School of Medicine - Shiraz University of Medical Sciences , Khosravi ، Amir Department of Ophthalmology - Poostchi Ophthalmology Research Center, School of Medicine - Shiraz University of Medical Sciences , Tanideh ، Nader Stem Cells Technology Research Center - Shiraz University of Medical Sciences
Abstract :
Human tears can be used as a noninvasive source of genetic materials and biomarkers in the prognosis and diagnosis of ocular and non-ocular diseases. The present protocol is a novel direct RNA extraction method from tears. This study aims to provide a suitable method for direct extraction of RNA from tears with high quality and quantity. In this study, we develop a TRIzol base protocol for direct RNA extraction from human tears. quality and quantity of extracted RNA measured by calculation of 260/280 UV absorption ratio using Nanodrop and real-time PCR. RNA was extracted with this modified method and a purified (260/280 UV absorption ratio between 1.8 to 2 and a high yield of total RNA, on average 95 μg, from tears was extracted. In conclusion, we developed an easy and suitable method for direct extraction of total RNA from tears with high quality and quantity.
Keywords :
RNA , Extraction method , Human tears , Biomarkers
Journal title :
Molecular Biology Research Communications
Journal title :
Molecular Biology Research Communications
