Title of article :
Effect of Cigarette versus Electronic Cigarette on the Expression of TERT, FGFR3, PTEN, P53, and VEGF in Rat Bladder
Author/Authors :
Afasri ، Rouhollah School of Medicine - Rafsanjan University of Medical Sciences , Mashhadi ، Rahil Department of Biology - Khatam University , Zareian Baghdadabad ، Leila Department of Biology - Medical Biotechnology Research Center - Yazd University , Mirzaei ، Akram Department of Biology - Islamic Azad University, Science and Research Branch , Zahmatkesh ، Parisa Department of Genetics - Islamic Azad University, Medical Branch , Mesbah ، Gholamreza AshianGanoTeb Biopharmaceutical Company - Golestan University of Medical Sciences , Keshavarz Pakseresht ، Behta Department of Genetics Biotechnology - School of Biological Science - Islamic Azad University, Varamin-Pishva Branch , Khajavi ، Alireza Student Research Committee, Faculty of Paramedical Sciences - Shahid Beheshti University of Medical Sciences , Khoshchehreh ، Mahdi Department of Pathology - University of California , Noori ، Maryam Student Research Committee, School of Medicine - Iran University of Medical Sciences , Rahimnia ، Ramin Department of Medical Nanotechnology - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences
Abstract :
Introduction This investigation aims to assess and compare the effect of cigarettes and e-cigarettes on the expression of TERT, FGFR3, PTEN, P53, and VEGF in rat bladder. Methods 60 Wistar rats were classified into three groups (10): Group A (Control), Group B: case (cigarette smoke), and Group C: case (E-cigarette smoke). The rats were exposed to cigarettes or e-cigarettes for 10 minutes. These 10 minutes were performed three times a day, and in total, the samples were exposed to cigarettes or e-cigarettes for 40 minutes a day with 1 hour rest for 16 weeks. Results Histopathological findings showed cigarette-induced hyperplasia and e-cigaretteinduced hyperemia and infiltration of inflammatory cells. The expression of FGFR3, TERT, and VEGF genes significantly increased and the expression of the PTEN gene significantly decreased in both cigarette and e-cigarette groups in both male and female rats compared to the control group but these changes were not significant between the two groups. The expression of P53 decreased in both groups, but the female rat in the e-cigarette significantly increased. Conclusions We found that both groups changed the expression of genes involved in the development of BC, but no differences were found two groups. Therefore, the e-cigarette is not an excellent alternative to cigarettes.
Keywords :
Bladder Cancer , E , cigarettes , Rat , Gene expression
Journal title :
Translational Research in Urology
Journal title :
Translational Research in Urology
