Biological activity of new heterocyclic compounds derived from chalcone

In this work, chalcones were synthesized by condensing ketone containing azo dye with aromatic aldehyde derivative (Vanillin) at room temperature and in a diluted ethanolic potassium hydroxide solution. The subsequent reaction of chalcone with urea, thiourea, hydrazine hydrate, and hydroxylamine hydrochloride led to the synthesis of novel heterocyclic derivatives including oxazine, thiazine, pyrazol, and isoxazole, respectively. After that, the effect of these compounds was studied on gram-positive (Staphylococcus Epiderimidis, pseudomonas, and gram-negative (Klebsiella pneumoniae and E.Coli) bacteria. The (FTIR, 1H-NMR, and 13C-NMR) spectroscopies were used to characterize each of the synthesized compounds.