Biosynthesis of copper oxide nanoparticles using Aspergillus niger extract and their antibacterial and antioxidant activities

the present study, nanoparticles of copper oxide were formed using a green approach. The Biosynthesis of CuO NPs was fabrication with the help of fungi which isolated from rot vegetables. The extract of Aspergillus niger fungi and Copper(II) chloride were used to synthesize bio-CuO NPs. Different techniques such as XRD (X-ray diffraction), FE-SEM (Field emission scanning electron microscopy), FTIR (Fourier transform infrared spectroscopy), and UV-Vis (UV–visible spectroscopy) were utilized to characterize and identify the biosynthesis of CuO NPs. The bioactivity of bio-CuO was screened against some types of pathogenic bacteria including Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Salmonella typhimurium via MHA (Muller Hinton agar) media. The results show that bio-CuO NPs have a potential effect against bacteria used in the current study and are more active toward Escherichia coli and Salmonella Typhimurium compared to other types. The antioxidant activity of bio-CuO was further studied in a dose-dependent manner. The obtained results show a positive effect of bio-CuO NPs and the activity increase when concentration is increased. Therefore based on the obtained results, the bio-CuO NPs have promising applications in the field of biomedical, industrial, and biological applications.