Entomopathogenic Nematodes Survey and Identification from Different Districts of Khyber Pakhtunkhwa, Pakistan

A survey was undertaken in six districts of Khyber Pakhtunkhwa i-e Chitral, Kohat, Haripur, Mardan, Swat, and Peshawar to collect entomopathogenic nematodes, Insect bait technique was used for Entomopathogenic nematodes collection. For this experiment, Galleria mellonella larvae were reared in the laboratory. When samples soil were collected from different regions of Khyber Pakhtunkhwa for nematodes collection G. mellonella larvae were left in sampling jar and were collected back after 2-3 days’ post application. Dead larvae were then put on the white trap and EPNs has then collected afterward. It is concluded on the basis of results that EPNs is positively recorded in grassy land, tomato field, forest land and poplar trees land in six selected districts of Chitral, Kohat, Haripur, Mardan, Swat, and Peshawar of Khyber Pakhtunkhwa. Furthermore, the EPNs is positively investigated to maximum level in Chitral, Peshawar, Mardan, Haripur, Swat, and Kohat respectively. The EPNs positive sample indication is directed that the environment of these districts are appropriate for EPNs presence.