Author/Authors :
serena, maria soledad national university of la plata - faculty of veterinary sciences, conicet (scientific research council) - department of virology, La Plata, argentina , metz, germán ernesto national university of la plata - faculty of veterinary sciences, conicet (scientific research council) - department of virology, la Plata, Argentina , lozada, maria ines national university of la plata - faculty of veterinary sciences,conicet (scientific research council) - department of pathology, La Plata, Argentina , aspitia, carolina gabriela national university of la plata - faculty of veterinary sciences - department of virology, la Plata, Argentina , nicolino, edgardo héctor national university of rosario - faculty of veterinary sciences - department of infectious diseases, Casilda, Argentina , pidone, claudio luis national university of rosario - faculty of veterinary sciences - department of infectious diseases, Casilda, Argentina , fossaroli, melisa national university of rosario - faculty of veterinary sciences - department of pathology, Casilda, Argentina , balsalobre, agustin national university of la plata, - conicet (scientific research council), cct la plata, La Plata, Argentina , quiroga, maria alejandra national university of la plata - faculty of veterinary sciences - department of pathology, La Plata, Argentina , echeverria, maria gabriela national university of la plata - faculty of veterinary sciences, conicet (scientific research council), cct la plata - department of virology, La Plata, argentina
Abstract :
Since Aujeszky`s disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.
