Author/Authors :
noruzi, somaye north khorasan university of medical sciences - faculty of medicine, student research committee - department of advanced sciences and technologies, Bojnurd, Iran , vatanchian, mehran north khorasan university of medical sciences - faculty of medicine - department of anatomical sciences, bojnurd, iran , azimian, amir north khorasan university of medical sciences - faculty of medicine - department of pathobiology and laboratory sciences, bojnurd, iran , niroomand, arash north khorasan university of medical sciences - faculty of medicine, student research committee - department of advanced sciences and technologies, Bojnurd, Iran , salarinia, reza north khorasan university of sciences - faculty of medicine - department of advanced sciences and technologies, bojnurd, iran , oroojalian, fatemeh north khorasan university of medical sciences - faculty of medicine - department of advanced sciences and technologies, bojnurd, iran
Abstract :
Background: The application of non-viral systems for delivering genes to cells is becominga very interesting issue, especially in the treatment of neoplasms such asBreast Cancer (BC). Polymer-based non-viral systems are safe and feasible genecarriers to be used in targeted cancer therapy. SALL4 gene encodes a transcriptionfactor and is overexpressed in some cancers.Methods: In this study, carboxyalkylated-PEI25 (25 kDa) was used to deliver plasmidsexpressing SALL4-siRNA into MCF-7 cells. DLS and AFM were applied to determinethe size of nanoparticles. The MTT method was used to assess cytotoxicity, and the efficiencyof transfection was confirmed both qualitatively and quantitatively. Finally,the effect of silencing SALL4 was investigated on the migration of MCF7 cells using thescratch test.Results: The results showed that transferring the SALL4-siRNA using PEI25G10C50 reducedthe expression of the corresponding transcription factor by 14 folds which attenuatedthe migration of MCF-7 cells by 58%.Conclusion: In conclusion, PEI25G10C50 can serve as an effective gene delivery systemfor treating BC by targeting SALL-4.
Keywords :
Breast neoplasms , Gene transfer techniques , MCF , 7 cells , Transcription factors
