Title of article :
Evaluation of mRNA Expression of CD244 and Its Adaptor Molecules in CD8+ T Cells in Acute Leukemia
Author/Authors :
Mohammadi ، Maryam Department of Immunology - School of Medicine - Mazandaran University of Medical Sciences , Asgarian-Omran ، Hossein Department of Immunology - School of Medicine,Gastrointestinal Cancer Research Center - Mazandaran University of Medical Sciences , Najfi ، Ahmad Department of Immunology - School of Medicine - Mazandaran University of Medical Sciences , Valadan ، Reza Molecular and Cell-Biology Research Center - Mazandaran University of Medical Sciences , Karami ، Hossein Thalassemia Research Center (TRC), Hemoglobinopathy Institute - Mazandaran University of Medical Sciences , Naderisoraki ، Mohammadd Thalassemia Research Center (TRC), Hemoglobinopathy Institute - Mazandaran University of Medical Sciences , Zaboli ، Ehsan Department of Hematology and Oncology - Gastrointestinal Cancer Research Center,Imam Khomeini hospital - Mazandaran University of Medical Sciences , Eslami ، Mohammad Department of Hematology and Oncology - Gastrointestinal Cancer Research Center,Imam Khomeini hospital - Mazandaran University of Medical Sciences , Tehrani ، Mohsen Department of Immunology - School of Medicine,Gastrointestinal Cancer Research Center - Mazandaran University of Medical Sciences
Abstract :
Background: This study investigated the role of the ICR, CD244, and its adapter molecules, in CD8^+ T cells in acute leukemia. Methods: Blood samples were obtained from 21 ALL and 6 AML patients and 20 control subjects. Relative gene expression of CD244, SAP , EAT-2, and LncRNA-GSTT1-AS1 were evaluated using qRT-PCR. Results: Expression of CD244, SAP, and EAT-2 were significantly lower in CD8^+ T cells from ALL patients than those from control subjects. Interestingly, the expression of SAP was much lower than that of CD244, indicating a lower ratio of SAP to CD244. Also, SAP expression was significantly lower in AML patients compared to the control group. Expression of LncRNA-GSTT1-AS1 showed no significant difference in ALL and AML patients compared to control subjects. Conclusion: The low SAP/CD244 expression ratio in CD8^+ T cells in ALL suggests an inhibitory role for CD244 in ALL.
Keywords :
Acute myeloid leukemia , CD244 , EAT , 2
Journal title :
Iranian Biomedical Journal(IBJ)
Journal title :
Iranian Biomedical Journal(IBJ)
