Nickel separation from human blood samples based on amine and amide functionalized magnetic graphene oxide nano structure by dispersive sonication micro solid phase extraction

Nickel (Ni) is toxic effect on human body and must be determined in human blood samples. In this study, Ni ions separated and preconcentrated from blood samples based on magnetic Fe3O4-supported amine/amide-functionalized graphene oxide (Fe3O4@A/A-GO) nanoparticles by dispersive sonication micro solid phase extraction (DS-μ-SPE). By procedure, 10 mg of Fe3O4@A/A-GO was dispersed in 10 mL of human blood samples with sonication for 5.0 min and then separated from liquid phase with magnetic accessory. The Ni ions was extracted based on amine/amide covalence bonding of Fe3O4@A/A-GO sorbent (Ni---: NH2). Then, the Ni ions back-extracted from Fe3O4@A/ A-GO in low pH with nitric acid (0.2 mL, 0.3 M) which was diluted with DW up to 0.5 mL and finally, was determined by ET-AAS (peak area). The LOD, linear range (LR), enrichment factor (EF) and absorption capacity (AC) were obtained 35 ng L^-1, 0.15 -7.2 μg L^-1, 19.8 and 131.6 mg g^-1, respectively. The method was validated by spiking samples.