Title of article :
Livestock, pets and humans as carriers of methicillin-resistant Staphylococcus aureus and comparative evaluation of two PCR protocols for detection
Author/Authors :
Sonowal ، Dharitree Department of Veterinary Public Health - College of Veterinary Science - Assam Agricultural University , Ghatak ، Sandeep Division of Animal and Fisheries Sciences - ICAR Research Complex for NEH Region , Barua ، Acheenta Gohain Department of Veterinary Public Health - College of Veterinary Science - Assam Agricultural University , Kandhan ، Srinivas Division of Animal and Fisheries Sciences - ICAR Research Complex for NEH Region , Hazarika ، Razibuddin Ahmed Department of Veterinary Public Health - College of Veterinary Science - Assam Agricultural University , Sen ، Arnab Division of Animal and Fisheries Sciences - ICAR Research Complex for NEH Region , Das ، Samir Division of Animal and Fisheries Sciences - ICAR Research Complex for NEH Region , Sonowal ، Sarat Department of Veterinary Public Health - College of Veterinary Science - Assam Agricultural University , Sharma ، Rajeev Kumar Department of Veterinary Microbiology - College of Veterinary Science - Assam Agricultural University , Tamuly ، Shantanu Department of Veterinary Biochemistry - College of Veterinary Science - Assam Agricultural University , Phukan ، Chimanjita Department of Microbiology - Gauhati Medical College - Srimanta Sankaradeva University of Health Sciences , Sharma ، Ajanta Department of Microbiology - Gauhati Medical College - Srimanta Sankaradeva University of Health Sciences , Hussain ، Poznur Department of Veterinary Public Health - College of Veterinary Science - Assam Agricultural University
Abstract :
Staphylococcus aureus are Gram positive bacteria known to acquire antibiotic resistance rapidly and pose a major challenge to clinicians worldwide. Infections by methicillin resistant Staphylococcus aureus (MRSA) are usually associated with increased mortality and prolonging of treatment. Samples (n = 706) from diverse sources (livestock, pets, animal handlers, human hospital) were collected and screened for the presence of MRSA by phenotypic and genotypic methods. The incidence of Staphylococcus aureus was greater in goats (42.00%; 28.20 - 56.80%, confidence interval [CI] 95.00%) followed by cattle (13.50%; 9.20 - 18.80%, CI 95.00%), humans (12.90%; 9.30 - 17.40%, CI 95.00%) and dogs (12.90%; 8.10 - 19.20%, CI 95.00%). Significantly higher incidence of MRSA was observed in dogs (65.00%; 40.80 - 84.60%, CI 95.00%), compared to other hosts namely cattle (48.00%; 26.50 - 64.30%, CI 95.00%), humans (35.00%; 20.20 - 52.50%, CI 95.00%) and goats (10.00%; 1.20 - 30.40%, CI 95.00%). All the S. aureus isolates were further screened for thermostable nuclease (nuc gene) by polymerase chain reaction (PCR). The incidence of nuc gene in cattle, dog, goat and human were found to be 3.30% (1.30 - 6.60%, CI 95.00%), 5.20% (2.30 - 9.90%, CI 95.00%), 28.00% (16.20 - 42.50%, CI 95.00%) and 9.10% (6.00 - 13.00%, CI 95.00%), respectively. Comparative evaluation of two PCR primers (mecA-162 and mecA-310) indicated the former one as more rational choice for detection of MRSA. Overall, the results of our study indicated possible risk of zoonotic transmission of MRSA from canines.
Keywords :
Cefoxitin , mecA , MRSA , nuc
Journal title :
Veterinary Research Forum
Journal title :
Veterinary Research Forum
