Theraupeutic Potency Of Benfotiamine Against Methotrexate- Induced Kidney Injury And Irisin Immunoreactivity

Objective: Benfotiamine (BFT) is an effective agent with anti-oxidative effects. In this study, the effects of BFT on methotrexate (MTX)-induced kidney injury and the immunoreactivity of irisin were investigated. Method: A total of 28 rats were separated into four equal groups. Control (no treatment was performed), BFT (50 mg/kg BFT was applied by oral gavage), MTX (20 mg/kg MTX was applied via intraperitoneal route), and MTX+BFT. At the end of the study, the removed kidney tissues were passed through routine histological follow-up series and embedded in paraffin blocks. For histopathological examination hematoxylin eosin, irisin and for caspase 3 streptavidin-biotin-peroxidase complex method was applied to sections taken from paraffin blocks. Total antioxidant status (TAS) and total oxidant status (TOS) status were determined with Rel Assay Kits. Results: MTX was found to have caused histopathological damage and a significant increase in irisin and caspase-3 immunoreactivity. Biochemically, significant increases in total oxidant status (TOS) and decreases in total antioxidant status (TAS) were determined in the animals given MTX. BFT treatment was found to ameliorate histopathological damage, significantly decrease TOS and caspase-3 immunoreactivity, increase TAS levels, and insignificantly decrease irisin immunoreactivity. Conclusion: Consequently, BFT exhibited protective effects in preventing kidney damage caused by MTX.