Author/Authors :
Zangeneh Motlagh ، Melika Department of Oral and Maxillofacial Pathology - School of Dentistry - Tehran University of Medical Sciences , Mahdavi ، Nazanin Department of Oral and Maxillofacial Pathology, Department of Pathology - School of Dentistry, Cancer Institute Hospital, Imam Khomeini Hospital (IKHC) - Tehran University of Medical Sciences , Miri-Lavasani ، Zohreh Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences , Aminishakib ، Pouyan Department of Oral and Maxillofacial Pathology, Department of Pathology - School of Dentistry, Cancer Institute Hospital, Imam Khomeini Hospital (IKHC) - Tehran University of Medical Sciences , Khoramipour ، Mahsa Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences , Kazem Arki ، Mandana Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences , Rezaei ، Niloufar Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences , Hossein-Khannazer ، Nikoo Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences , Vosough ، Massoud Department of Regenerative Medicine - Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology - Academic Center for Education, Culture and Research (ACECR)
Abstract :
Objective: Surgery and chemotherapy are the most common therapeutic strategies proposed for oral squamous cell carcinoma (OSCC). However, some of the disadvantages associated with the current methods like unwanted side effects and poor drug response lead the scientist to seek for novel modalities and delivery approaches to enhance the efficacy of treatments. The study aimed to assess the effectiveness of disulfiram (DSF)-loaded Niosomes on cancerous phenotypes of the OSCC cells. Materials and Methods: In this experimental study, an optimum formulation of DSF-loaded Niosomes was developed for the treatment of OSCC cells to reduce drug doses and improve the poor stability of DSF in the OSCC environment. The design expert software was utilized to optimize the particles in terms of size, polydispersity index (PDI), and entrapment efficacy (EE). Results: Acidic pH increased the release rate of DSF from these formulations. The size, PDI, and EE of Niosomes were more stable at 4°C compared to 25°C. The results indicated that DSF-loaded Niosomes could induce apoptosis (P=0.019) in the OSCC cells compared to the control group. Moreover, it could reduce colony formation ability (P=0.0046) and also migration capacity of OSCC cells (P=0.0015). Conclusion: Our findings indicated that the application of proper dose of DSF-loaded Niosomes (12.5 μg/ml) increases apoptosis, decreases colony formation capacity and declines the migration ability of OSCC cells.
Keywords :
Disulfiram , Drug Delivery , Niosome , Oral Squamous Cell Carcinoma
