Isolation, Structure Elucidation, and Standardization of Suberosin and Glycyrrhizin from Ferulago trifida L. and Glycyrrhiza glabra L.

Natural products currently often considered as a starting point in drug discovery, followed by synthetic reforms to increase bioavailability and alleviate side effects. Approximately, fifty percent of the approved drugs by the U.S. Food and Drug Administration (FDA) are inspired by natural products. Glycyrrhizin is a well-known anti-inflammatory component that has been shown to prolong the coagulation time of thrombin and fibrinogen and to increase the duration of plasma recalcification. Suberosin, also known as 7-methoxy-6-prenylcoumarin, belongs to the class of coumarin compounds. The purpose of this article is to purify, identify, and standardize glycyrrhizin and suberosin, from Glycyrrhiza glabra L. and Ferulago trifida L. First, two plants were pulverized and then extracted using a percolator. The extracts were then divided into different fractions by column chromatography and mixed with thin-layer chromatography. Finally, we obtained pure compounds after using column chromatography and thin-layer chromatography. 1H-NMR, 13C-NMR have been used for the purified compounds and the HPLC method was used to standardize these compounds. Glycyrrhizin and Suberosin were extracted from Glycyrrhiza glabra L. and Ferulago trifida L respectively and 1H-NMR 13C-NMR techniques approved the structure of these two compounds, also, standardization of these compounds was correctly done by HPLC.