Title of article :
A Small Regulatory RNA, Rli82, Is Involved in the Motility and Pathogenicity of Listeria monocytogenes
Author/Authors :
Ji ، Chunhui College of Animal Science and Technology - Shihezi University , Li ، Nengxiu College of Animal Science and Technology - Shihezi University , Jiao ، Jian College of Animal Science and Technology - Shihezi University , Sun ، Yaoqiang College of Animal Science and Technology - Shihezi University , Zuo ، Yufei College of Animal Science and Technology - Shihezi University , Huang ، Xin Institute of Animal Science and Veterinary Research - Xinjiang Academy of Agricultural and Reclamation Science , Huang ، Xiaoxing College of Animal Science and Technology - Shihezi University , Li ، Zhiyuan College of Animal Science and Technology - Shihezi University , Li ، Yaling College of Animal Science and Technology - Shihezi University , Leng ، Qingwen College of Animal Science and Technology - Shihezi University , Cai ، Xuepeng State Key Lab of Veterinary Etiological Biology - Lanzhou Veterinary Research Institute - Chinese Academy of Agricultural Sciences , Meng ، Qingling College of Animal Science and Technology - Shihezi University , Qiao ، Jun College of Animal Science and Technology - Shihezi University
Abstract :
Background: Listeria monocytogenes(LM) is a facultative intracellular pathogen that causes food-borne infections in humans and animals. To invade and multiply within host cells, LM utilizes various strategies to precisely modulate its gene expression and to adapt to the in vivoenvironment. Objectives: To investigate the regulatory roles of Rli82sRNA in the motility and pathogenicity of LM EGD-e. Methods: The Rli82gene knock-out mutant strain, LM-Δ Rli82, and the complementation strain, LM-Δ Rli82/ Rli82, were constructed using homologous recombination technology, and their motility and virulence, respectively, were determined. Moreover, the potential target mRNA regulated by Rli82was predicted using TargetRNA2 software, and then the interaction between the target mRNA and Rli82was verified by the two-plasmid reporter system. Results: The results showed that the motility of LM-Δ Rli82was significantly increased at 25°C, facilitated by the production of more flagella than LM EGD-e and LM-Δ Rli82/ Rli82. Furthermore, LD 50in LM-Δ Rli82-infected mice was significantly increased as compared to LM EGD-e and LM-Δ Rli82/ Rli82, suggesting that the virulence of LM was weakened when the Rli82gene was deleted. In addition, the mRNA level of flaAwas not significantly elevated, but flaAprotein was significantly higher in LM-Δ Rli82than in LM EGD-e and LM-Δ Rli82/ Rli82, suggesting that Rli82might modulate the translation of flaAmRNA at the post-transcriptional level. Conclusions: Taken together, our findings for the first time revealed that Rli82sRNA might be involved in the modulation of the expression of flaAprotein, thereby influencing the mobility and pathogenicity of LM.
Keywords :
Listeria monocytogenes , Regulatory sRNA , Rli82 , Motility , Pathogenicity
Journal title :
Jundishapur Journal of Microbiology (JJM)
Journal title :
Jundishapur Journal of Microbiology (JJM)
