Title of article :
Histopathological Assessment of Testicular Tissues from Mice with Xenografted Human Triple-Negative Breast Cancer
Author/Authors :
Davoudi ، Armaghan Electrical Engineering Faculty - Islamic Azad University, Najafabad Branch , Chamaneh ، Mahdieh Medical School - Shandong University , Sung Chieng ، Teodor Medical School - Shandong University , Kaviani ، Fahimeh Department of Pharmacology - AshianGanoTeb Biopharmaceutical Company - Golestan University of Medical Sciences , Moghiminejad Davarani ، Sogand Faculty of Veterinary Medicine - Islamic Azad University, Baft Branch , Iranmanesh ، Mohamad Amin Faculty of Veterinary Medicine - Islamic Azad University, Baft Branch , Omid ، Reza Urology Research Center - Tehran University of Medical Sciences , Ehsani ، Jalal Department of Food - Drug and Natural Products Health Research Centre - Golestan University of Medical Sciences , Amerian ، Razieh Department of Pathology - Taleghani Hospital - Golestan University of Medical Sciences , Kashani ، Kian Faculty of Veterinary Medicine - Islamic Azad University, Baft Branch , Mesbah ، Gholamreza Department of Pathology - AshianGanoTeb Biopharmaceutical Company - Golestan University of Medical Sciences
Abstract :
Introduction: Mammary tumors are a common cancer in human but little is known about their histopathological characteristics and effects on other organs. Understanding tumor effects on testicular tissue has implications for developing mouse models of human breast cancer.Methods: Nude mice were divided into two groups, with 5 mice implanted with mammary tumor cells (experimental group) and 5 mice serving as controls. After tumor growth, histopathological analysis of the tumors and testes was performed using hematoxylin and eosin staining. Several features were qualitatively scored in tumor and testis sections including mitosis, necrosis, angiogenesis, inflammation, hyperemia, and atrophy.Results: Tumors from experimental mice showed significantly higher mitotic rate, necrosis, angiogenesis, inflammation, and atrophy compared to controls. Multiple differences were observed in testis pathology compared to a control group. Experimental tumors displayed more aggressive qualities.Conclusion: This study demonstrates that mammary tumors generated in nude mice exhibit elevated pathological characteristics tied to aggressiveness, including proliferation and cell death, compared to control mice. Notably, tumor growth affected testis histology including atrophy and hyperemia. These results provide insights into using this mouse model of breast cancer and establish a baseline for pathological features of experimental mammary tumors versus healthy tissues.
Keywords :
breast cancer , MDA , MB , 231 xenograft model , nude mice
Journal title :
Translational Research in Urology
Journal title :
Translational Research in Urology
