Title of article :
The Effect of Mesenchymal Stem Cells Derived-Conditioned Media in Combination with Oral Anti-Androgenic Drugs on Male Pattern Baldness: An Animal Study
Author/Authors :
Kamali-Dolat Abadi ، Majid Department of Tissue Engineering and Applied Cell Sciences - School of Advance Medical Science and Technology - Shiraz University of Medical Sciences , Yousefi ، Gholamhossein Department of Pharmaceutics - School of Pharmacy - Shiraz University of Medical Sciences , Dehghani ، Farzaneh Department of Anatomical Sciences - Histomorphometry and Stereology Research Center, School of Medicine - Shiraz University of Medical Sciences , Alizadeh ، Ali Akbar Department of Tissue Engineering and applied cell science - School of Advanced Medical Sciences Technologies - Shiraz University of Medical Sciences , Jangholi ، Abolfazl Department of Biology - Faculty of Science - Razi University , Moadab ، Mohammad Amin Department of Biology - Zand Institute of Higher Education , Naseh ، Maryam Histomorphometry and Stereology Research Center - Shiraz University of Medical Sciences , Parsa ، Shima Transplant Research Center - Shiraz University of Medical Sciences , Nasiri ، Golara Department of Tissue Engineering and Cell Therapy - School of Advanced Technologies in Medicine - Shiraz University of Medical Sciences , Azarpira ، Negar Transplant Research Center - Shiraz University of Medical Sciences , Dianatpour ، Mehdi Department of Medical Genetics - Stem Cell Technology Research Center, School of Medicine - Shiraz University of Medical Sciences
Abstract :
Objective: Androgenetic alopecia (AGA) is a prevalent form of hair loss, mainly caused by follicular sensitivity toandrogens. Despite developing different anti-androgen treatment options, the success rate of these treatments hasbeen limited. Using animal models, this study evaluated the therapeutic effects of umbilical cord (UC) stem cellconditioned media (CM) combined with oral anti-androgens for hair regeneration.Materials and Methods: In this experimental study, Poloxamer 407 (P407) was used as a drug carrier forsubcutaneous testosterone injection. AGA models were treated with oral finasteride, oral flutamide, and CMinjections. Samples were thoroughly evaluated and compared using histological, stereological, and molecularanalyses.Results: Injecting CM-loaded hydrogel alone or combined with oral intake of anti-androgens improved hair regeneration.These treatments could promote hair growth by inducing hair follicles in the anagen stage and shortening the telogenand catagen phases. Furthermore, the combination treatment led to an upregulation of hair induction gene expressionwith a downregulation of inflammation genes.Conclusion: Through a reduction in inflammation, injection of CM-loaded hydrogel alone or combined with oral intakeof anti-androgens induces the hair cell cycle with regeneration in damaged follicles. Hence, this could be a promisingtherapeutic method for AGA patients.
Keywords :
Androgenetic Alopecia , Conditioned Media , Finasteride , Flutamide , Mesenchymal stem cells
Journal title :
Cell Journal (Yakhteh)
Journal title :
Cell Journal (Yakhteh)
