Title of article
Comparing the Biological Properties of Double-Stranded DNA Extracted from Human and Porcine Placenta and Salmon Sperm
Author/Authors
Proskurina ، Anastasia Institute of Cytology and Genetics - Russian Academy of Sciences, Siberian Branch , Nikolin ، Valeriy Institute of Cytology and Genetics - Russian Academy of Sciences, Siberian Branch , Popova ، Nelly Institute of Cytology and Genetics - Russian Academy of Sciences, Siberian Branch , Varaksin ، Nikolay JSC “Vector-Best” , Ryabicheva ، Tatiana JSC “Vector-Best” , Ershova ، Elizaveta Research Centre for Medical Genetics , Kostyuk ، Svetlana Research Centre for Medical Genetics , Leplina ، Olga Research Institute of Fundamental and Clinical Immunology , Ostanin ، Alexandr Research Institute of Fundamental and Clinical Immunology , Chernykh ، Elena Research Institute of Fundamental and Clinical Immunology , Bogachev ، Sergey Institute of Cytology and Genetics - Russian Academy of Sciences, Siberian Branch
From page
577
To page
589
Abstract
Background: Double-stranded fragmented extracellular DNA is a participant, inducer, and indicator of various processes occurring in the organism. When investigating the properties of extracellular DNA, the question regarding the specificity of exposure to DNA from different sources has always been raised. The aim of this study was to perform comparative assessment of biological properties of double-stranded DNA obtained from the human placenta, porcine placenta and salmon sperm. Methods: The intensity of leukocyte-stimulating effect of different dsDNA was assessed in mice after cyclophosphamide-induced cytoreduction. The stimulatory effect of different dsDNA on maturation and functions of human dendritic cells and the intensity of cytokine production by human whole blood cells was analyzed ex vivo. The oxidation level of the dsDNA was also compared. Results: Human placental DNA exhibited the strongest leukocyte-stimulating effect. DNA extracted from human and porcine placenta exhibited similar stimulatory action on maturation of dendritic cells, allostimulatory capacity, and ability of dendritic cells to induce generation of cytotoxic CD8+CD107a+ T cells in the mixed leukocyte reaction. DNA extracted from salmon sperm stimulated the maturation of dendritic cells, while having no effect on their allostimulatory capacity. DNA extracted from human and porcine placenta was shown to exhibit a stimulatory effect on cytokine secretion by human whole blood cells. The observed differences between the DNA preparations can be caused by the total methylation level and are not related to differences in oxidation level of DNA molecules. Conclusions: Human placental DNA exhibited the maximum combination of all biological effects.
Keywords
Cytokines , Cytotoxic T cells , Dendritic cells , Double , stranded DNA.
Journal title
Reports of Biochemistry and Molecular Biology (RBMB)
Journal title
Reports of Biochemistry and Molecular Biology (RBMB)
Record number
2759124
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