Biocompatibility Analysis of Mouse Spleen-Derived Extracellular Matrix

Background Objectives: This study aimed to construct a decellularized mouse spleen scaffold and evaluate its cellular compatibility in vitro using murine bone marrow-derived mesenchymal stem cells (BM-MSCs). Materials Methods: A combination of physical, chemical, and enzymatic treatments was employed for mouse spleen decellularization. These included multiple freeze-thaw cycles, the ionic detergent sodium dodecyl sulfate (SDS), and enzymatic trypsin. Histological and scanning electron microscopy analyses were conducted up to 7 days post-culture to assess the impact of decellularization and cellular adaptation to the spleen scaffolds. Results: Histological studies revealed the attachment and penetration of BM-MSCs into the scaffolds on days 5-7 following cell seeding. Furthermore, cell migration into the scaffold was observed 5 days after the seeding process. Conclusion: The decellularization approach utilized in this study proved to be effective and biocompatible, supporting the preservation and proliferation of BM-MSCs. These findings indicate its potential for spleen tissue engineering applications.