Reverse Flow Injection Method for Estimation of Hesperidin and Naringenin in Supplements with 3-Methyl-2-Benzothiazolinone Hydrazone Hydrochloride

The detection of Hesperidin (HSP) and Naringenin (NAR) in dietary supplements and human urine samples is recommended using a reversed flow-injection analysis (rFIA) technology that is quick, accurate, and highly responsive. Using 3-methyl-2-benzothiazolinone Hydrazone Hydrochloride (MBTH) in the presence of sodium periodate as an oxidizing agent, the suggested approach uses oxidative coupling procedures. With a peak absorbance for HSP at 507 nm and for NAR at 495 nm, a red complex is the resultant product. An extensive analysis of the physical and chemical elements influencing the reaction has been conducted. Between 2 and 150 µg/mL, the calibration curve showed a steady and proportionate relationship. It was found that the detection limits for NAR and HSP were 0.911 µg/mL and 0.856 µg/mL, respectively. For HSP and NAR, the relative standard deviation (RSD) was found to be 0.79% and 0.64%, respectively. Drug quantitation was effectively accomplished using the recommended methods.