Title of article
Inactivated herpes simplex virus-1 vaccine formulated in aqueous and alcoholic extracts of propolis boosts cellular and IgG responses
Author/Authors
Mojarab ، Sanaz Advanced Therapy Medicinal Product (ATMP) Department - Breast Cancer Research Center, Motamed Cancer Institute - Academic Center for Education, Culture, and Research (ACECR) , Karimi ، Pegah Department of Biochemistry - Faculty of Basic Sciences - Islamic Azad University, Central Tehran Branch , Shahbazzadeh ، Delavar Venom and Biotherapeutics Molecules Laboratory, Medical Biotechnology Department - Biotechnology Research Center - Pasteur Institute of Iran , Moghbeli ، Majid Department of Biology - Islamic Azad University, Damghan Branch , Pooshang Bagheri ، Kamran Venom and Biotherapeutics Molecules Laboratory, Medical Biotechnology Department - Biotechnology Research Center - Pasteur Institute of Iran , Beig Parikhani ، Arezoo Venom and Biotherapeutics Molecules Laboratory, Medical Biotechnology Department - Biotechnology Research Center - Pasteur Institute of Iran , Dehghan ، Rada Venom and Biotherapeutics Molecules Laboratory, Medical Biotechnology Department - Biotechnology Research Center - Pasteur Institute of Iran , Zafari ، Ehsan Advanced Therapy Medicinal Product (ATMP) Department - Breast Cancer Research Center, Motamed Cancer Institute - Academic Center for Education Culture, and Research (ACECR) , Moravej ، Amir Department of Microbiology - Razi Vaccine and Serum Research Institute - Agricultural Research, Education and Extension Organization (AREEO) , Pouriyayevali ، Mohammad Hassan COVID-19 National Reference Laboratory, Department of Arboviruses and Viral Hemorrhagic Fevers, National Reference Laboratory - Pasteur Institute of Iran , Mirtalebi ، Faranak Department of Microbiology - Islamic Azad University, Zanjan Branch , Pakjoo ، Mahdi Advanced Therapy Medicinal Product (ATMP) Department - Breast Cancer Research Center, Motamed Cancer Institute - Academic Center for Education, Culture, and Research (ACECR) , Yazdani ، Shaghayegh Department of Microbiology - Faculty of Advanced Science and Technology, Faculty of Medicine - Islamic Azad University, Tehran Medical Sciences , Abdollahpour-Alitappeh ، Meghdad Cellular and Molecular Biology Research Center - Larestan University of Medical Sciences , Mahdavi ، Mehdi Advanced Therapy Medicinal Product (ATMP) Department - Breast Cancer Research Center, Motamed Cancer Institute - Academic Center for Education, Culture, and Research (ACECR)
From page
1209
To page
1213
Abstract
Objective(s): In this study, the adjuvant activity of aqueous and alcoholic extracts of propolis was examined on the inactivated herpes simplex virus-1 (HSV-1). Materials and Methods: BALB/C mice were administered with inactivated (HSV-1; the KOS strain) plus alcoholic and aqueous extracts, followed by assessment of the cellular and humoral immune responses. Results: Alcoholic and aqueous extracts, as an adjuvant, revealed a significant increase in lymphocyte proliferation and cytotoxic T lymphocyte (CTL) responses versus the HSV-1 group. In addition, HSV-1 plus alcoholic extract showed a remarkable increase in IFN-γ cytokine and IFN-γ/IL-4 ratio. On the other hand, both alcoholic and aqueous extracts in the HSV-1 vaccine suppressed the IL-4 cytokine response as compared with the HSV-1 vaccine. In addition, HSV-1 plus alcoholic extract showed a significant increment in IgG1, IgG2a, and IgG2b isotypes as compared with the HSV-1 vaccine. Conclusion: Propolis extracts seem to modulate the immune response against inactivated HSV-1 model and can be used as a suitable vaccine adjuvant or a component of a complex adjuvant against infectious diseases.
Keywords
Adjuvant , HSV , 1 , Propolis , Th1 , Vaccine potency
Journal title
Iranian Journal of Basic Medical Sciences
Journal title
Iranian Journal of Basic Medical Sciences
Record number
2759860
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