Purified Compound of the Seeds of Carica Papaya Loaded mPEG-PLA Polymeric Nanomicelle (MCPI-PNs) for Reproductive Studies in Albino Male Rats

This study compares the characterized MCP I-loaded methoxy poly (ethylene glycol)-poly (lactic acid) polymeric nanomicelles (MCPI-PNs) of different sizes and doses to find out the maximum antireproductive effects with purified compound of seeds of Carica papaya. The prepared MCPI-PNs consisting of six groups (n=36), viz., vehicle treated control (group 1), alone MCP I @ 10 mg/animal/day (group 2), 46.03 nm sized blank mPEG-PLA nanomicelles @ 0.3 mL/animal/day (group 3), 100 nm sized MCPI-PNs (1.5, 3.0 and 4.5 µg/animal/day) (group 4), 129 nm sized MCPI-PNs (4.5, 9.0 and 13.5 µg/animal/day) (group 5) and 166 nm sized MCPI-PNs (13.5, 27.0 and 45.0 µg/animal/day) (group 6) were orally treated for a period of 30 days in albino male rats. Animals of groups 2, 4, 5 and 6 showed significantly altered sperm parameters. However, the sperm concentration maximally dropped in group 2 and 4 (low dose and mid dose), respectively, indicating the range of oligozoospermia to severe oligozoospermia in these groups. The fertility declined to zero percent in groups 2 and 4 (low dose), whereas all animals were 100% fertile in rest of groups. Histopathological studies of testes exhibited sloughing and eruption of germ cells with inhibition of spermatogenesis in groups 2 and 4, whilst some conspicuous alterations in spermatogenesis was observed in groups 5 and 6 when compared to groups 1 and 3. Based on the above observations, the group 4 (100 nm sized MCPI-PNs) at low dose regimen (1.5 µg/animal/day) exhibited maximum antifertility effects in male albino rats.