Monophosphate as Eminent Ligand to Bind Ce(IV)/EDTA Complex for Site-selective DNA Hydrolysis

By the use of oligonucleotides bearing a terminal monophosphate, 10-base gap was formed in substrate DNA and the monophosphates were placed at the edge of gap. Upon the treatment of these systems with Ce(IV)/EDTA complex, the gapsite was selectively hydrolyzed. The monophosphates remarkably promoted both the selectivity and the efficiency of scission.