Title of article
Bone Marrow and Peripheral Blood Mononuclear Cell Phenotype Changes after Cultivation and Autologous Infusion in Patients with Primary Biliary Cholangitis
Author/Authors
Saipiyeva ، Dana Transplantation and Research Department - National Scientific Medical Center , Askarov ، Manarbek Transplantation and Research Department - National Scientific Medical Center , Jafari ، Nazanin PerciaVista R D Co. Shiraz , Zhankina ، Rano Department of Urology and Andrology - Astana Medical University , Heath ، Paul R. Department of Neuroscience - Sheffield Institute for Translational Neuroscience (SITraN) - University of Sheffield , Kozina ، Larissa Clinical and Diagnostic Laboratory - National Scientific Medical Center , Boltanova ، Alyona Clinical and Diagnostic Laboratory - National Scientific Medical Center , Omarbekov ، Ardak Angiosurgery and Plastic Surgery Department - Astana Medical University , Ilyassov ، Nurbek Department of Surgery - National Scientific Medical Center , Tuganbekov ، Turlybek Angiosurgery and Plastic Surgery Department - Astana Medical University , Mussin ، Nadiar M. West Kazakhstan Marat Ospanov Medical University , Kaliyev ، Asset A. West Kazakhstan Marat Ospanov Medical University , Sultangereyev ، Yerlan West Kazakhstan Marat Ospanov Medical University , Rahmanifar ، Farhad Department of Basic Sciences - School of Veterinary Medicine - Shiraz University , Mahdipour ، Mahdi Stem Cell Research Center - Tabriz University of Medical Sciences , Bakhshalizadeh ، Shabnam Murdoch Children’s Research Institute , Shirazi ، Reza Department of Anatomy - School of Biomedical Sciences , Tanideh ، Nader PerciaVista R D Co. Shiraz , Tamadon ، Amin Department of Natural Sciences - West Kazakhstan Marat Ospanov Medical University
From page
559
To page
572
Abstract
Background: Primary biliary cholangitis (PBC) is a condition affecting the liver and immune system. In this study, the impact of autologous bone marrow-derived mononuclear cell (BM-MNC) transplantation on PBC patients was investigated. Methods: Sixteen eligible PBC patients participated at the National Scientific Medical Center in Astana, Kazakhstan, between 2017 and 2022, and BM-MNCs were harvested from their anterior iliac crest. After isolating and cultivating the BM-MNCs, they were infused back into the patient’s peripheral veins. Changes in BM-MNC and peripheral blood mononuclear cell (PB-MNC) phenotypes were assessed before and after a 24-hour cultivation period and 72 hours post-transplantation. We monitored liver function parameters over 6-month intervals and conducted flow cytometry analysis to assess CD markers on BM-MNCs before and after cultivation and PB-MNCs before and after transplantation. Statistical analysis included the Friedman test for liver parameters and the Wilcoxon signed-rank test for BM-MNC and PB-MNC comparisons.Results: Our findings revealed significant reductions in liver function tests after multiple transplantations. Flow cytometry analysis before and after a 24-hour culture and autologous BM-MNC infusion revealed the expansion of specific cell populations, with significant increases in CD3+, CD4+, CD16+, CD20+, CD25+, CD34+, CD105+, CD73+, СD117+, and CD34+populations, while CD4+25+, CD34+105+, and CD4+FOXP3+ populations decreased. Interestingly, a contradictory finding was observed with a decrease in bone marrow CD34+105+ cell lines (P=0.03) alongside an increase in peripheral CD34+105+ population (P=0.03).Conclusion: In summary, our study shows that BM-MNC transplantation in PBC patients leads to changes in immune cell populations and liver function. These findings suggest potential therapeutic applications of BM-MNC transplantation in managing PBC and offer insights into the dynamics of immune cells associated with this treatment approach.
Keywords
Primary biliary cholangitis , Leukocytes , mononuclear , Bone Marrow , Peripheral blood stem cells
Journal title
Iranian Journal of Medical Sciences (IJMS)
Journal title
Iranian Journal of Medical Sciences (IJMS)
Record number
2768756
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