Title of article
Bioinformatic and Phylogenetic Investigation of WRKY Genes Involved in Drought Stress in Camelina sativa Plant
Author/Authors
Seyed Hassan Pour ، Maryam Department of Genetic and Plant Breeding - Faculty of Agriculture - Shahid Chamran University of Ahvaz , NejadSadeghi ، Leila Department of Genetic and Plant Breeding - Faculty of Agriculture - Shahid Chamran University of Ahvaz , Kahrizi ، Danial Department of Agricultural Biotechnology - Faculty of Agriculture - Tarbiat Modares University , Shobbar ، Zahra Sadat Department of Systems Biology - Agricultural Biotechnology Research Institute of Iran (ABRII) - Agricultural Research, Education and Extension Organization (AREEO)
From page
65
To page
79
Abstract
Camelina (Camelina Sativa) is a hexaploid dicotyledonous plant from the Brassicaceae family, which is very similar to the Arabidopsis. The number of camelina chromosomes is 40=2n. WRKY transcription factors are one of the most important gene families in plants that play an important role in regulating growth and development and response to various stresses. In this research, 224 WRKY genes were identified in the camelina plant by searching the database, and the chromosomal position, gene length, and conserved motifs were identified in the camelina plant based on the Arabidopsis. Also, in this study, in order to validate the research, 2 genes WRKY8 and WRKY57 under drought stress were investigated by the qPCR method. The results indicated that both the above-mentioned genes were strongly expressed under drought stress conditions in tolerant varieties compared to normal conditions, but the trend was opposite in sensitive varieties. This study has provided acceptable and valuable information for studying the evolution and function of the WRKY gene family in camelina.
Keywords
Abiotic stress , Brassicaceae , qRT , PCR , Transcriptional factors , WRKY
Journal title
Agrotechniques in Industrial Crops
Journal title
Agrotechniques in Industrial Crops
Record number
2769249
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