Title of article :
Molecular Detection of Mutations in gyrA, gyrB, parC, and parE Genes in the Quinolone Resistance Determining Region Among Pseudomonas aeruginosa Isolated From Burn Wound Infection
Author/Authors :
Akhlaghi ، Fatemeh Student Research Committee, School of Medicine - Guilan University of Medical Sciences , Nikokar ، Iraj Department of Microbiology - School of Medicine - Guilan University of Medical Sciences , Mojtahedi ، Ali Department of Microbiology - School of Medicine - Iran University of Medical Sciences , Mobin ، Mohammadreza Department of Surgery - Guilan University of Medical Science , Atrkar Roshan ، Zahra Student Research Committee, School of Medicine - Guilan University of Medical Sciences , Karampour ، Moslem Student Research Committee, School of Medicine - Guilan University of Medical Sciences
From page :
89
To page :
96
Abstract :
Background and Aim: The prevalence of fluoroquinolone resistance among Pseudomonas aeruginosa isolates, particularly in burn wound infections, has been increasing. The primary cause of ciprofloxacin resistance is attributed to genetic alterations in the quinolone resistance determinant region (QRDR). In this study, we evaluated the antimicrobial resistance profile and QRDR gene mutations in both ciprofloxacin-resistant and non-resistant strains of P. aeruginosa derived from burn wound patients. Materials and Methods: A total of 300 samples were collected from patients with burn wound infections in Guilan, Iran. The isolates were identified as P. aeruginosa, and drug susceptibility tests were conducted using the agar disk diffusion method. DNA extraction and polymerase chain reaction (PCR) analysis were performed for the amplification and sequencing of gyrA, gyrB, parC, and parE genes in the QRDR region. Results: Resistance to Tobramycin, Gentamicin, Piperacillin, Ciprofloxacin, Ceftazidime, and Amikacin was observed in 59.32%, 55.08%, 51.69%, 50.84%, 30.50%, and 26.27% of the isolates, respectively. Forty-two (35.59%) isolates were multi-drug-resistant (MDR). The sequencing results in the QRDR region showed that the majority of mutations were in the gyrA gene, with 85.71% of these mutations being the substitution of threonine with isoleucine (Thr-83 Ile) in ciprofloxacin-resistant strains. Conclusion: The results of this study indicate that mutations in the gyrA (gyrase gene) are a significant mechanism of resistance to fluoroquinolones. Identifying these mutations can aid in the detection of fluoroquinolone-resistant isolates and simplify treatment challenges by selecting the appropriate antibiotic.
Keywords :
Antibiotic Resistance , Ciprofloxacin , Resistant , QRDR Mutation , Pseudomonas aeruginosa ,
Journal title :
Iranian Journal of Medical Microbiology
Journal title :
Iranian Journal of Medical Microbiology
Record number :
2769357
Link To Document :
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