Title of article
Intranasal administration of human adipose-derived stem cell-conditioned media ameliorates cognitive performance in a rat model of Alzheimer’s disease
Author/Authors
shaker ، Amir Department of Anatomy - School of Medicine - Iran University of Medical Sciences , Parsaei ، Houman Department of Anatomy - Nervous System Stem Cells Research Center, Faculty of Medicine - Semnan University of Medical Sciences , amini ، Naser Neuroscience Research Center, School of Medicine - Iran University of Medical Sciences , Nobakht ، Maliheh Department of Anatomy - School of Medicine, Cellular and Molecular Research Center - Iran University of Medical Sciences , fahanik babaei ، Javad Electrophysiology Research Center - Tehran University of Medical Science , Dadseresht ، Ali Department of Anatomy - School of Medicine - Iran University of Medical Sciences , Seidkhani ، Elham Department of Anatomy - School of Medicine - Iran University of Medical Sciences , Eftekharzadeh ، Mina Department of Anatomy - School of Medicine, Neuroscience Research Center - Iran University of Medical Sciences
From page
43
To page
55
Abstract
Introduction: Alzheimer’s disease (AD) is an age-related disorder, characterized by the gradual loss of memory and cognitive function owing to neuronal damage and brain shrinkage. This study aimed to investigate how intranasal injection of human adipose-derived stem cell-conditioned media (hADSC-CM) ameliorates cognitive performance and affects the level of estrogen receptor beta (Erβ) in the hippocampus of rats in an AD model. Methods: A total of 32 male rats were divided into four groups, including the control, AD model, hADSC-CM, and vehicle groups. The Morris water maze was used to assess the animals’ behavioral changes. Moreover, Nissl and Thioflavin-S staining were performed to evaluate the histology of the hippocampus. Immunohistochemistry was also carried out to evaluate the expression level of Erβ. Results: The intranasal injection of hADSC-CM improved the rats’ cognitive performance by reducing the number of dark cells and beta-amyloid plaques in the hippocampus in the AD model. Besides, the intranasal injection of hADSC-CM increased the level of Erβ in this model. Conclusion: The present findings indicated that the intranasal injection of hADSC-CM ameliorated cognitive function. Amyloid plaques and dark cells also diminished in the CA1 area of the hippocampus. Moreover, the expression level of ERβ increased. It can be concluded that hADSC-CM has significant treatment benefits for AD in rats.
Keywords
Alzheimer’s Disease , Estrogen receptors , Human adipose , drive stem , Cell therapy , Cognitive performance
Journal title
Physiology and Pharmacology
Journal title
Physiology and Pharmacology
Record number
2771575
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