Title of article
Properties of Compounds Citral and Geraniol on Trichomonas gallinae: Activity in Vitro and Cytotoxicity
Author/Authors
Baccega ، Bruna Parasitology Laboratory, Department of Microbiology and Parasitology - Federal University of Pelotas , Fenalti ، Juliana Monteli Parasitology Laboratory, Department of Microbiology and Parasitology - Federal University of Pelotas , de Mello ، Alexia Brauner Parasitology Laboratory, Department of Microbiology and Parasitology - Federal University of Pelotas , Islabão ، Yan Wahast Parasitology Laboratory, Department of Microbiology and Parasitology - Federal University of Pelotas , Birmann ، Paloma Taborda Neurobiotechnology Research Group, Postgraduate Program in Biotechnology - Technological Development Center - UFPel , Neis ، Alessandra Postgraduate Program in Biotechnology - Technological Development Center - UFPel , De Giacomti ، Marjorie Parasitology Laboratory, Department of Microbiology and Parasitology - Federal University of Pelotas , Monteiro ، Francielle Liz Laboratory of Virology and Immunology - Faculty of Veterinary - UFPel , Hubner ، Silvia De Oliveira Laboratory of Virology and Immunology - Faculty of Veterinary - UFPel , Vaucher ، Rodrigo De Almeida Research Laboratory in Biochemistry and Molecular Biology of Microorganisms (LaPeBBioM) - UFPel , Farias ، Nara Amélia da Rosa Parasitology Laboratory, Department of Microbiology and Parasitology - Federal University of Pelotas , Norouzi ، Roghayeh Department of Pathobiology - Faculty of Veterinary Medicine - University of Tabriz , Siyadatpanah ، Abolghasem Infectious Diseases Research Center - Gonabad University of Medical Sciences , Oliveira ، Camila Belmonte Parasitology Laboratory, Department of Microbiology and Parasitology - Federal University of Pelotas
From page
440
To page
447
Abstract
Background: Avian trichomoniasis is an important disease that causes bird mortality, both wild and captive, around the world. This study evaluated the in vitro cytotoxicity, antioxidant, and antiparasitic activity of citral (3.7-Dimetil-2.6-octadienal) and geraniol (trans-3.7-Dimetil-2.6-octadien-1-ol) against Trichomonas gallinae trophozoites. Methods: In vitro assays were conducted at the Laboratory of Protozoology and Entomology (LAPEN) at the Federal University of Pelotas (UFPel), Brazil in 2019 using tests with 106 parasites and citral and geraniol at concentrations ranging from 10 to 100 μM and four controls: NC (culture medium and trophozoites), MTZ (trophozoites plus 100 μM of metronidazole), and TW (trophozoites plus vehicles used for solubilizing derivatives (0.01% Tween). Results: The citral (60 μM) and geraniol (50 μM) concentrations reduced the trophozoitesʼs viability by 100%. The molecular docking experiment demonstrated that citral and geraniol might inhibit a hydrogen enzyme for T. gallinae survival. Conclusion: The major compounds of lemongrass have potential antitrichomonal activity against T. gallinae in vitro.
Keywords
Trichomonas gallinae , Citral , Geraniol , Cytotoxicity , Molecular docking
Journal title
Iranian Journal of Parasitology (IJP)
Journal title
Iranian Journal of Parasitology (IJP)
Record number
2773255
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