Title of article
Correlation of 2,4-DDT with CDH1 Gene Promoter Methylation in Gastric Cancer
Author/Authors
Ashrafi ، Mohammad reza Department of Clinical Biochemistry - Neuroscience Research Center, Institute of Neuropharmacology, Applied Cellular and Molecular Research Center, Afzalipour School of Medicine - Kerman University of Medical Sciences , Asadi Karam ، Gholamreza Department of Clinical Biochemistry - Applied Cellular and Molecular Research Center, Afzalipour School of Medicine - Kerman University of Medical Sciences , Darvishmoghddam ، Sadif Gastroenterology and Hepatology Research Center - Kerman University of Medical Sciences , Abolhassani ، Moslem Department of Clinical Biochemistry - Afzalipour School of Medicine, Applied Cellular and Molecular Research Center - Kerman University of Medical Sciences , Iranpour ، Maryam Pathology Department - Kerman faculty of medical science , Sepehri ، Zahra Department of Biochemistry Medical Genetics - University of Manitoba
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1
To page
7
Abstract
Background: Methylation alterations of tumor suppressor gene promoters are important epigenetic changes in gastric cancer. CDH1 encodes a protein (cadherin1) with essential roles in cell-cell adhesion. In this study, the association of organochlorine pesticide (OCP) serum levels with the methylation profile of this gene was investigated in gastric cancer, intestinal metaplasia (IM), and functional dyspepsia (FD) patients. Methods: Gastric cancer (n = 34), IM (n = 8), and FD (n = 48) patient serums were analyzed for the determination of OCP levels by gas chromatography. The methylation status of the CDH1 gene promoter was examined by the methylation-specific PCR (MSP) method. Immunohistochemistry (IHC) was used to confirm the reduced protein expression of this gene in methylated samples. Results: Our findings revealed significant hypermethylation of the CDH1 gene promoter and its reduced expression in gastric cancer patients compared with IM and FD patients. Furthermore, there was a significant association between CDH1 promoter hypermethylation and 2,4-DDT (OR = 1.183; 95% CI = 1.001–1.398; P = 0.048) serum levels in gastric cancer patients. Conclusion: Our results suggest an association between 2,4-DDT OCP levels in gastric cancer patient serums with CDH1 gene promoter hypermethylation. Additionally, this gene promoter’s methylation may play a role in the progression of pre-cancerous IM towards gastric cancer.
Keywords
organochlorine pesticides , CDH1 , DNA methylation , Gastric cancer , intestinal metaplasia
Journal title
Journal of Kerman University of Medical Sciences (JKMU)
Journal title
Journal of Kerman University of Medical Sciences (JKMU)
Record number
2774060
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