Effect of temperature and pH on primary metabolic and biomass productivity culture in Euglena sp.

Euglena is a microalga with the potential to be used as a renewable energy source. The biofuel-making potential is present in Euglena species biomass s proteins, lipids, and carbohydrates. Therefore, optimizing microalgal growth under various physiological conditions is crucial to obtaining more biomass. In this study, Euglena sp. was cultivated on medium Cramer-Myers (CM) and subjected to various temperatures and acidities. Euglena sp. cultures were optimized at different pH levels, including 2.5, 3.5, and 5.5, and at 29 and 32°C. Then, treatments were evaluated on the culture s pace of cell development, total biomass, and amount of carbohydrates, protein, and lipids. Based on the results, Euglena sp. at pH 5.5 and 29°C had the optimal growth rate, biomass, carbohydrate, protein, and fat content compared to the other treatments. In a pH 5.5 at 29°C, the average biomass was 0.382±0.173 g/L, and the resulting concentrations of protein, carbohydrates, and lipids of 0.288±0.12, 0.201±0.052, and 0.182±0.083 g/L, respectively.