Title of article :
Diagnostic potential of lncRNAs-ANRIL and MIAT in the blood of patients with cerebral venous thrombosis
Author/Authors :
Zayani ، Zoofa Department of Biology - Islamic Azad University, Zarghan Branch , Hooshmandi ، Etrat Clinical Neurology Research Center - Shiraz University of Medical Sciences , Borhani-Haghighi ، Afshin Clinical Neurology Research Center - Shiraz University of Medical Sciences , Rahimi ، Mousa Laboratory of Basic Sciences - Shiraz University of Medical Sciences , Ostovan ، Vahid Reza Clinical Neurology Research Center - Shiraz University of Medical Sciences , Fadakar ، Nima Clinical Neurology Research Center - Shiraz University of Medical Sciences , Tabrizi ، Reza Noncmmunicable Diseases Research Center - Fasa University of Medical Sciences , Bayat ، Mahnaz Clinical Neurology Research Center - Shiraz University of Medical Sciences , Hojati ، Shamim Department of Biology - Islamic Azad University, Zarghan Branch , Gharbi ، Negin Department of Biology - Islamic Azad University, Zarghan Branch , Mahmoudinasab ، Hamideh Department of Biology - Islamic Azad University, Zarghan Branch
Abstract :
Background: Long non-coding ribonucleic acids (lncRNAs) have been implicated as possible circulating stroke indicators. This study focused on the expression status of antisense non-coding ribonucleic acid in the INK4 locus (ANRIL) and myocardial infarction associated transcript (MIAT) in patients with cerebral venous thrombosis (CVT).Methods: In this study, fifty patients with CVT and one hundred age/gender-matched individuals as controls were included. The circulating levels of ANRIL and MIAT in the first 24 hours after admission were evaluated using the quantitative real-time polymerase chain reaction (RT-PCR) method. We compared the expression levels of ANRIL and MIAT between patients and controls using the independent two-sample t-test. Subgroup analysis was used to investigate the association of lncRNAs with clinical characteristics in patients with CVT. Receiver operating characteristic (ROC) curve analyses were conducted to evaluate the diagnostic value of two lncRNAs in patient assessment.Results: The relative expression of lncRNAs ANRIL and MIAT significantly decreased in patients compared to the control. ANRIL and MIAT were shown as potential markers for discriminating patients with CVT from the healthy controls with an area under the curve (AUC) of 0.98 and 0.99, respectively.Conclusion: For the first time, we found down-regulation and diagnostic potential of lncRNAs-ANRIL and MIAT in the blood of patients with CVT.
Keywords :
Venous Thrombosis , Stroke , Long Non , Coding RNA , Myocardial Infarction Associated Transcript , Gene Expression
Journal title :
current journal of neurology
Journal title :
current journal of neurology
