6-Amino-6-deoxychitosan. Preparation and Application as Plasmid Vector in COS-1 Cells

6-Amino-6-deoxychitosan was prepared from 6-deoxy-6-halo-N-phthaloylchitosan via 6-azidation. The product has high stereoregularity because of the effective and regioselective reactions. To evaluate 6-amino-6-deoxychitosan as a gene transfer agent, transfection efficiencies of its complexes with plasmid DNA in COS-1 cells were examined.