Genes expression of cellulase enzyme in four Aspergillus species

Production of cellulase is of great importance for industrially processes and developing the metabolic microbes used widely in different fields. Aspergillus, as one of fungal genera, serve to produce many enzymes such as cellulase. Twenty-three isolates of fungi belong to four species of Aspergillus were obtained from central lab veterinary medicine in Basra, Iraq and screened for maximum cellulolytic activity. Screening of fungal species was done on Petri plate containing carboxymethyl cellulase growth media. Among 23 isolates of fungi, 20 fungal isolates revealed cellulolytic activity and three depicted zero cellulase activity. All isolates of A. niger showed strong secretion of cellulolytic activity on agars followed by A. fumigatus, with two isolates of A. flavus and one isolateof A. terreus revealed zero cellulytic activity. The results of relative gene expression of Aspergillus species to five cellulase genes cbhb, exogluconase, endogluconase A, endogluconase B, endogluconase C and control B actin gene revealed over expression of A. niger compared to other species.