Effect of ethidium bromide on hematological, biochemical, and immunological paramours in mice

This study aimed to investigate the effect of Ethidium bromide (EB) exposure on hematological, biochemical and immunological parameters of mice. A totalof 60 BALB/c female mice were acclimated and divided into 6 groups, including: a healthy control group (GC) and 5 experimental groups given orally different doses of EB; GA (3ml), GB (9ml), GD (15ml), GE (21ml) and GF (30ml) for 1 month. Based on the results, MON was decreased significantly in all experimental groups; while, tRBCs, Hb, HCT, LYM, BAS, PLT, RDW and MPV reduced significantly in GD, GE and GF. Significant increases were observed in values of MCV, MCH, tWBCs and NEU, particularly in GD, GE and GF groups. However, no significant change was detected in MCHC and EOS. In biochemical factors, the HDL and MIP-1a were decreased significantly in GB, GD, GE and GF; whereas, increases in ALT, ALP, MDA, BUN, BIL and LDH were found significantly in GD, GE and GF groups. Also, AST, Cr and LDL were raised significantly in all experimental groups. For GLU, significant increases were recorded in GB and GD, while significant decreases in GF. For immunological parameters, the IgG was increased significantly in all experimental groups; whereas, a significant reduction in IgM was detected in GD. This study showed that the severe EB poisoning had obvious abnormalities in hematological, biochemical, and immunological parameters in mice. The identification of powerful prognostic markers is important for the management of patients with EB poisoning in emergency settings; therefore, further studies are required to clarify the mechanisms of toxicity and the adverse role of EB on tissues and organs.