Antibiotic resistance and plasmid profiling of Pseudomonas aeruginosa isolated from some ruminants in Basrah, Iraq

This work aimed to determine the antibiotic-resistant patterns of Pseudomonas aeruginosa isolates obtained from clinical, healthy, and environmental samples from some ruminants (cows, and sheep). A total of 200 P. aeruginosa were obtained, and 52 isolates resisted all antibiotics used in the antibiotic sensitivity test. The antibiotic-resistant pattern showed that P. aeruginosa had high resistance (100%) to ampicillin, ceftazidime, gentamycin, ciprofloxacin, piperacillin, tobramycin, imipenem, amikacin, streptomycin, levofloxacin, rifampin, tetracycline, trimethoprim, ofloxacin, carbenicillin, penicillin, and nalidixic acid and had low resistant to colistin and fosfomycin. The plasmid profile was carried out on 12 selected multidrug-resistant (MDR) isolates that were resistant to more classes of antibiotics. All strains were found to possess plasmid bands. Five of the strains had 3 plasmid bands, 4 strains 2 plasmid bands and 3 strains possessed a single band. The sizes of the plasmids among P. aeruginosa isolates were 735, 1400 and 3000bp. All the strains that had plasmids were resistant to gentamycin, ciprofloxacin, piperacillin, tobramycin, imipenem, carbenicillin and tetracycline.