Evaluating the antibacterial and biofilm activity of Pseudomonas aeruginosa isolated from dog’s wound infections

This study aimed to evaluate the antibacterial and biofilm activity of P. aeruginosa isolated from dog wound infections. One hundred samples were collected from February to December 2022 including 28 male and 72 female. Pseudomonas aeruginosa isolates were identified by cultural characteristics, biochemical tests. Thirty P.aeruginosa isolates (30%) were identified. The biofilm producing ability of P. aeruginosa isolates was evaluated by using pre-sterilized 96 well polystyrene microtiter plates. The results showed that out of 20 P. aeruginosa, 1 (5%) was non-adherent and weak, 18 (90%) strong biofilms. The susceptibility test of P. aeruginosa isolates toward 10 different antibiotics were carried out by Kirby-Bauer method. The results revealed that all isolates were resistant to Ciprofloxacin (CIP), Cefotaxime (TX) and Gentamycin (CN) 43.33%,follow by 46.66% resistant to Levofloxacin (LEV), 63.33% to Ceftraxone (CRO), 50% to Azithrofomycin(AZM), 96.66% P. aeruginosa to Ampicillin (AM) and Erythromycin (E), 26.66% to Ceftaziaime (AZ), and 6.66% to Amikacin (AK). The results of the current study showed that the majority of isolates with high resistance to antibiotics had the ability to form biofilms.