Histopathological alternation of the liver and spleen of mice treated with Ag nanoparticles

Ag Nanoparticles are recently among the most utilized nanomaterials due to their unique characteristics. These characteristics make them desirable to numerous industries but, also may contribute to their potential toxicity. The toxicity of colloidal Ag NPs solution on the liver and spleen of mice was studied. This solution was administrated with two concentrations of 0.5 and, 1ppm to females by intraperitoneal injection and another over two periods of 30 and 45 days. Twenty-five female mice weighing about 25-30g were divided into five groups, a control group (GC), G1 was injected with 0.5ppm of AgNPs for 30 days, G2 was injected with 1ppm for 30 days, G3 was injected with 0.5ppm for 45 days and G4 group injected with 1ppm for 45 days. Animals were then sacrificed and their liver and spleen were removed, preserved, processed, sectioned, and stained. Slides were examined with Lieca fluorescent microscope. The results revealed tangible histological changes in the liver and spleen in the G1 group. However severe deteriorations (hemorrhage, fibrosis, necrosis, and deposition of amyloid protein in hepatic vein) were observed in the rest. In conclusion, Ag Nps caused severe damage to the architecture of the liver and spleen even when utilized with less concentration.