Migration of Human Wharton’s Jelly Stem Cells Seeded Onto the Chorioallantoic Membrane Into the Chick Liver

Background: Developing chicken embryo culture techniques provides methods for manipulating chicken embryos. In this study, the cell-based approach investigated human Wharton›s jelly stem cells (HWJSCs) proliferation and migration into internal embryonic chick tissue.Methods: Wharton’s jelly cells were cultured on an acellular dermal matrix (ADM) and seeded on the chorioallantoic membrane (CAM) of a 4-day-old chicken embryo. Using an RT-PCR assay with the COLA-I human gene, molecular analysis was performed on chicken liver tissue after hatching to confirm the presence of these cells.Results: The chicken liver tissue from Wharton’s jelly stem cells/ADM sample tested positive for the human COLA-I gene. The COLA-I gene sequence analysis shows this sequence belongs to the COLA-I gene in humans. The cells cultured on CAM can migrate and proliferate in the liver of a chicken embryo.Conclusion: These findings suggest that human cells cultured on the CAM can appear in the liver of chickens. However, further investigations are necessary to confirm this finding.