Enhancement of xylanase enzyme production by induced gamma radiation mutation of Trichoderma

Xylanase enzymes produced by Trichoderma afroharzianum have significant industrial applications, including animal feed, food, biofuel, and textile industries. Creating novel sources of Trichoderma strains using induced gamma irradiation mutation can increase enzyme production. According to this, Co-60 gamma irradiation has been used to develop a mutant strain of T.afroharzianum. T.afroharzianum mutants were isolated, and qualitative and quantitative screening were used to evaluate the production of the extracellular enzymes with wheat bran waste as a substrate. The best T.afroharzianum mutant strain was identified using the DNA barcoding method. The highest xylanase activities were observed in the superior mutant of T.afroharzianum NAS107-M82, which is approximately 3.3 times higher than its parent strain. The electrophoretic pattern of proteins showed that synergistically, the exo-glucanase I, endo-glucanase III, and the xylanase I enzymes hydrolyzed the wheat bran. This study collectively highlights the diverse potential of gamma-radiated T. afroharzianum mutant-derived xylanases in various industrial and agricultural applications, showcasing their importance in enzyme production.