Dietary Effects of major plantain ( i Plantago major /i L.) extraction on hematological, biochemical indices-indices and innate immune system in Common carp ( i Cyprinus carpio /i ) fingerlings

Major plantain (Plantago major) is belonged to Plantaginaceae family, traditionally it was used commonly in folk medicine for skin poultices on wounds, sores, or insect stings. This study was investigated the effect of different levels of major plantain extraction on hematological, biochemical and immunological indices in common carp (Cyprinus carpio). Common carp fingerlings (N=240, 18.69±2.09g) were randomly distributed in 12 tanks (3 treatments in 3 replicates). The fingerlings were fed with 0 (as control), 0.1, 0.5 and 1% major plantain extract for 45 days. At the end of the experiment, blood samples were taken from the caudal vein, and some hematological (RBC, HCT, Hb, MCV, MCH, MCHC, WBC, Lymphocyte, Monocyte, Neutrophil) factors, serum biochemical (Glucose, AST, ALT, ALP) and immunological (Total Ig, Globulin, Albumin, Total protein) indices-indices were evaluated. There were no significant differences in RBC, HCT, MCV, ALP, AST, ALT, Total protein, Albumin and total Immunoglobulin concentrations and also Monocytes, lymphocytes and neutrophils between treatments fed different levels of major plantain extract and the control group (P 0.05). Hb, MCH, MCHC, globulin levels in treatment fed with 0.1% major plantain extract was remarkably higher than other groups (P 0.05). Glucose level in treatments fed with 0.1 and 1% major plantain extract were not remarkably different from each other (P 0.05), but were significantly higher than two other groups (P 0.05). Finally, due to positive effect of major plantain extract at the level of 0.1% on some hematological indices-indices and the immune responses in common carp, it can be suggested to use major plantain extract to promote immune system in common carp.