Toxic effects of bintaro (cerbera manghas) seed extract on aedes aegypti mosquito

The use of chemicals in disease vector control has had a negative impact on the quality of environmental health. To reduce this impact, this study aimed to evaluate the effect of different bintaro fruit seed extracts (n-hexane and 96% ethanol) on the mortality of Ae. aegypti mosquitoes. A total of 1120 samples of 3-5-day-old adult Ae. aegypti mosquitoes were used in this study, with 3 repetitions. The research design was experimental, using a post-test-only controlled group design. Mosquito mortality was recorded after 24 hours of observation, while the toxicity effect of the spray on adult mosquitoes was recorded at 3-minute intervals for 20 minutes. The results showed that the administration of n-hexane and 96% ethanol extract solutions of C. manghas fruit seeds with different concentrations had different impacts on mosquito mortality. LC50 and LC90 for the n-hexane solution were 11,840 (118,400 ppm) and 26,646 (262,460 ppm), while the 96% ethanol extract solution was 5,207 (52,070 ppm) and 10,436 (104,360 ppm) in 100 mL of distilled water. An ANOVA test on n-hexane and 96% ethanol extracts of C. manghas seeds showed a significant difference between the two types of solutions. The yield of the maceration method using 96% ethanol solvent showed higher results than the n-hexane yield, with a significant value of 0.001 (p 0.05). There is a significant difference between each concentration of bintaro fruit seed extract and the mortality of Ae. aegypti mosquitoes.