Title of article :
Diphtheria Toxin Repressor (dtxR) Gene-Based Genetic Diversity of Corynebacterium diphtheriae Isolated in Jakarta, Indonesia, 2018–2019
Author/Authors :
Yasmon ، Andi Department of Microbiology - Faculty of Medicine, Cipto Mangunkusumo Hospital - Universitas Indonesia , Evriarti ، Paulina Rosa Biomedical Science Graduate Program - Faculty of Medicine - Universitas Indonesia , Rosanna ، Yeva Department of Microbiology - Faculty of Medicine, Cipto Mangunkusumo Hospital - Universitas Indonesia
Abstract :
Background and Aim: In Indonesia, diphtheria cases caused by Corynebacterium diphtheriae are still occurring. One of the causes is probably the diphtheria toxin repressor (dtxR) gene, which influences toxin expression. In this study, the gene was characterized to determine the mutations that affect the DtxR protein. Materials and Methods: The dtxR genes of 10 C. diphtheria strains isolated in Jakarta were amplified by conventional PCR. The PCR products were directed to DNA sequencing by using overlapping primers. Results and Conclusion: No mutations showed amino acid changes. We propose that the DtxR protein is conserved among bacterial strains isolated from Jakarta.
Keywords :
Corynebacterium diphtheriae , dtxR , Mutation , Phylogeny tree , Corynebacterium diphtheriae , dtxR ,
Journal title :
Iranian Journal of Medical Microbiology
Journal title :
Iranian Journal of Medical Microbiology
