Novel Direct Preparation of n-Butyl 2-Amino-2-deoxy-(beta)-D-glucopyranoside from Chitosan and n-Butanol Using Biocatalyst

n-Butyl 2-amino-2-deoxy-(beta)-D-glucopyranoside (C4GlcN) was prepared by the novel direct transglycosylation reaction of chitosan and n-butanol using the resting cells of Penicillium funiculosum KY616 as the enzyme source of exo-(beta)-Dglucosaminidase. Using this strain, a versatile synthetic intermediate, C4G1cN, was prepared in a yield of 210 mg/g chitosan when chitosan and n-butanol were incubated at a temperature of 30 °C and pH 4 for 2 d.