Calcium Phosphate Formation on Highly-oriented Collagen Fibrils

Calcium phosphate formation on collagen fibrils was studied. Collagen fibrils deposited from a collagen solution were insolubilized by UV-irradiation. The collagen fibrils looked to be precipitated and arranged in one direction in a narrow space due to convection stream from bottom to top of test tube. Highly-oriented collagen fibrils precipitated were soaked in simulated body fluid (SBF) solution at 36.5 °C. After immersion of SBF solution at 36.5 °C for 3 days, calcium phosphate was formed on insolubilized collagen fibrils. In contrast, no calcium phosphate was formed on collagen fibrils without insolubilization treatment.