Layer-by-Layer Assembly of Low Molecular Weight Dye/Enzyme Composite Thin Films for Biosensor Appilcation

The multilayer films of low molecular weight dye methylene blue (MB) and horseradish peroxidase (HRP) were fabricated by layer-by-layer assembly through electrostatic force. MB was pre-absorbed with anionic polyelectrolyte poly (sodium-p-styrenesulfonate) (PSS). The MB/HRP multilayer film-modified Au electrode functioned as a biosensor for H2O2.