In situ Monitoring of Poly(L-glutamine)-Bound dsDNA Selection on a Quartz-Crystal Microbalance

We applied a highly sensitive quartz-crystal microbalance as a device of dsDNA in vitro selection. When poly(Lglutamine)-immobilized QCM was employed, both the selection and evaluation processes could be monitored in situ as mass changes, and the dsDNAs having the continuous sequences of 35 AT base-pairs were selected after the 4th cycle from a random dsDNA pool.