SPECTROPHOTOMETRIC DETERMINATION OF CEFPROZIL IN PHARMACEUTICAL DOSAGE FORMS, IN URINE AND IN THE PRESENCE OF ITS ALKALINE INDUCED DEGRADATION PRODUCTS

Four simple, rapid and accurate methods for spectrophoto-metric determination of cepfrozil are presented. Method I and II depend on measuring first derivative peak-trough amplitude of the drug in 0.1 N HCl (D1a) and in phosphate buffer pH 7(D1b), respectively. Method III and IV are based on measuring the absorbance difference (?A) and first derivative difference (?D1) of buffered cefprozil solutions against its acidic solutions as blanks. The proposed methods are applied for the determination of the drug in pure and dosage forms with good accuracy and precision. Among the previously mentioned methods; D1a, D1b and ?D1 are used for the determination of cefprozil in urine. These methods have been successfully applied for the determination of cumulative amounts of cefprozil in urine following an oral dose of 500 mg to a human male volunteer. Moreover, by using D1a method and applying the zero-crossing technique, both the intact drug and its alkaline induced degradation products could be determined in presence of each other without interference. The proposed methods proved to be accurate and reproducible.