Optimisation of a culture medium containing fish silage for -lysine production by Corynebacterium glutamicum

In a first step the effects of 10 components of a culture medium designed for -lysine production were evaluated with a 210–6 factorial design. Among them, glucose, fish silage, and ammonium sulphate showed a significant effect. In a second step, an orthogonal–central composite experimental design and response surface methodology was performed with five from the 10 initial compounds. The determination coefficient (R2) of the fitted second-order model was 0.990. -lysine production with the optimised medium increased 2.6 times as compared with the original medium.