Title of article :
Bioactivity assay of extracts from Calocedrus macrolepis var. formosana bark
Author/Authors :
Louis Kuoping Chao، نويسنده , , Kuo-Feng Hua، نويسنده , , Hsien-Yeh Hsu، نويسنده , , Yu-Chang Su، نويسنده , , Shang-Tzen Chang، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2006
Abstract :
Alcoholic extracts from bark of Calocedrus macrolepis var. formosana Florin (Cupressaceae) were extracted successively using n-hexane, dichloromethane, ethyl acetate, 1-butanol and water, which gave 34.8%, 34.1%, 24.1%, 3.3% and 3.7% soluble fractions, respectively. Antioxidation activity of these fractions by DPPH assay and dissimilar IC50 values of the DPPH showed that ethyl acetate fraction had the best antioxidant activity; its IC50 was 2.6 μg/ml. Analyses of the composition and anti-inflammatory activity of the subfractions from n-C6H14 fraction showed that the T3 and H5ppt had the best anti-inflammatory activity in LPS-stimulated murine macrophage J774A. 1 cells, respectively; moreover, their major constituent was sugiol (T3 37.1%, H5ppt 81.1%), which at dosages of 10 μg/ml inhibited proIL-1β protein production completely. Furthermore, the T1 also exhibited anti-inflammatory activity, and its major constituent was ferruginol (above 85.6%).
Keywords :
Anti-inXammatory capacity , Extracts , antioxidant activity , Calocedrus macrolepis var. formosana
Journal title :
Bioresource Technology
Journal title :
Bioresource Technology